pins DNA molecules in place
Technology Research News
DNA molecules are extremely long and are
usually coiled up. Getting them to lie flat and straight and stay in place
has challenged many a lab technician, whether in studying their role as
the blueprints for life or using them as building blocks of nanotechnology.
A research team based at Delft University of Technology has developed
a technique to position, stretch out and pin down DNA molecules with nanometer-scale
The technique combines the previously developed process of DNA combing,
which stretches out DNA molecules, with photolithography, and also takes
advantage of the affinity DNA molecule ends have for polystyrene.
"Single molecules of DNA can be stretched, patterned and directed site-specifically
onto an arbitrary surface [when] molecular combing... is used in combination
with current lithographic techniques," said Dionne C. G. Klein, a graduate
student at Leiden University.
The researchers demonstrated the technique by using electron beam lithography
to produce a grid of polystyrene lines two microns wide and 17.5 microns
apart on a gold-covered silicon wafer. Polystyrene is used to make hard
plastics and Styrofoam.
They submerged the wafer in water containing molecules of the DNA from
a bacteria-infecting virus. Because the ends of the DNA molecules are
hydrophobic, they bind to the polystyrene, which is also hydrophobic.
The researchers then comb the DNA by slowly drawing the wafer out of the
water. The surface tension exerts a strong enough force to pull the loose
ends of the DNA molecules straight but not strong enough to pull the fixed
ends loose from the polystyrene. "The single-stranded DNA ends bind to
the [polystyrene] and the meniscus of the solution forces the coiled DNA
to stretch," said Klein.
The free end of each molecule, which can stretch to more than 20 microns
long, then attaches to the next polystyrene line in the grid.
The method keeps the DNA molecules lined up. The researchers found that
the DNA molecules did not attach both ends to the same polystyrene line.
The researchers also fixed stretched DNA molecules to unpatterned polystyrene
Positioning, stretching and pinning DNA molecules makes it easier for
researchers to examine them with atomic force and scanning tunneling microscopes.
It is also a step toward eventually using DNA molecules to make integrated
circuits much smaller than today's computer chips.
"Patterned DNA on a substrate can serve as a template for wires and for
two-and three-dimensional nanoscale devices," said Klein.
Klein's research colleagues were Leonid Gurevich, Jorg W. Janssen and
Leo P. Kouwenhoven of Delft University of Technology and Jeffrey D. Carbeck
and Lydia L. Sohn of Princeton University. They published the research
in the April 16, 2001 issue of the journal Applied Physics Letters.
The research was funded by the Foundation for Fundamental Research on
Matter in the Netherlands, the Exploratory Research for Advanced Technology
program in Japan and the National Science Foundation.
TRN Categories: Biological, Chemical, DNA and Molecular
Story Type: News
Related Elements: Technical paper, "Ordered stretching of
single molecules of deoxyribose nucleic acid between microfabricated polystyrene
lines," Applied Physics Letters, April 16, 2001
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